Journal
BIOMATERIALS
Volume 30, Issue 27, Pages 4629-4638Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.05.061
Keywords
Connective tissue; Mesenchymal stem cell; Stem cell; Bone tissue engineering; Growth factors; Adhesion molecule
Funding
- National Institute of General Medical Sciences (NIH) [NIH RO1 AR42997, NIH RO1 AG024980, NIH RO1 GM59870, NIH DE019523]
- National Institute of General Medical Sciences (NIGMS) Cell Migration Consortium
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Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeutic outcomes might be improved by using the scaffold to deliver growth factors that promote the early stages of cell function in the graft. Signaling by the epidermal growth factor receptor (EGFR) plays a role in cell survival and has been implicated in bone development and homeostasis. Providing epidermal growth factor (EGF) in a scaffold-tethered format may sustain local delivery and shift EGFR signaling to pro-survival modes compared to soluble ligand. We therefore examined the effect of tethered EGF on osteogenic colony formation from human bone marrow aspirates in the context of three different adhesion environments using a total of 39 donors. We found that tethered EGF, but not soluble EGF, increased the numbers of colonies formed regardless of adhesion background, and that tethered EGF did not impair early stages of osteogenic differentiation. (C) 2009 Elsevier Ltd. All rights reserved.
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