Journal
BIOMATERIALS
Volume 30, Issue 12, Pages 2284-2290Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.01.002
Keywords
Magnetic nanocomposite; Biosensor; Enzyme; Electrochemistry
Funding
- NNSF of China [20675064]
- Ministry of Education of China [708073]
- Chongqing Municipal Key Laboratory on Luminescence and Real-Time Analysis [2006CA8006]
- Chongqing Science and Technology Commission [2008AB5081]
- NSF of Chongqing [CSTC-200413134149, 200504100]
- High Technology Project Foundation of Southwest University [XSGX02]
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The preparation, characterization and application of a three-layer magnetic nanoparticle composed of an Fe3O4 magnetic core, a Prussian Blue (PB) interlayer and a gold shell (it can be abbreviated as Au-PB-Fe3O4) for an ultrasensitive and reproducible electrochemical immunosensing fabrication were described for the first time in this work. With the employment of the Au-PB-Fe3O4 nanoparticle, a new signal amplification strategy was developed based on bienzyme (horseradish peroxidase and glucose oxidase) functionalized Au-PB-Fe3O4 nanoparticles for an electrochemical immunosensing fabrication by using Carcinoembryonic antigen (CEA) and a-fetoprotein (AFP) as model systems, respectively. The experiment results show that the multilabeled Au-PB-Fe3O4 nanoparticles exhibit satisfying redox electrochemical activity and high enzyme catalysis activity, which predetermine their utility in high sensitivity antibody detection schemes. Furthermore, this immunosensor could be regenerated by simply using an external magnetic field which ensured a reproducible immunosensor with high sensitivity. (C) 2009 Elsevier Ltd. All rights reserved.
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