The gene-expression and phenotypic response of hFOB 1.19 osteoblasts to surface-modified titanium and zirconia

The osteoblastic cell-line hFOB 1.19 with the potential to proliferate and differentiate revealed that cellular differentiation is not affected by material and roughness on newly developed zirconia implant materials. Materials under investigation were surfaces machined titanium (Ti-m), modified titanium (TiUnite (R)), machined zirconia (TZP-A-m), modified zirconia (ZiUnite (R)), machined alumina-toughened zirconia (ATZ-m) and modified alumina-toughened zirconia (AU-mod). After surface description by scanning electron microscopy (SEM) and atomic force microscopy (AFM), cellular proliferation (EZ4U, Casy 1) and differentiation were examined after clays 1, 3, 7,14, 21, and 28. Osteogenic differentiation was visualized by alkaline phosphatase staining, mineralization assay (alizarin red) and by expression analysis (RT-PCR) of bone- and extracellular matrix-related genes. Proliferation on rough surfaces was reduced oil both titanium and zirconia. Cell-attachment and cytoskeleton organization documented by confocal laser scanning microscopy (CLSM) elucidated attenuated cell attachment within the first 4 h to be the reason for impaired proliferation. A specific up-regulation of m-RNAs in an early event (RUNX2, NELL-1, RUNX3, and BMP7) and a late event (Integrin B3) could be observed oil TiUnite (R) and ZiUnite (R). For titanium all up-regulation of IBSP and Integrin 131 Could be described at day B1. In total, differentiation was neither affected by material nor by roughness. (C) 2008 Elsevier Ltd. All rights reserved.