Journal
BIOMATERIALS
Volume 30, Issue 20, Pages 3397-3404Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.03.027
Keywords
AFM; Microfabrication; Protein patterning; Cortical neurons; Self-assembled monolayers; Neural network
Funding
- NSEC [DMR-0425880]
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We demonstrate that Atomic Force Microscopy nanolithography can be used to control effectively the adhesion, growth and interconnectivity of cortical neurons on Au surfaces. We demonstrate immobilization of neurons at well-defined locations on Au surfaces using two different types of patterned proteins: 1) poly-D-lysine (PDL), a positively charged polypeptide used extensively in tissue culture and 2) laminin, a component of the extracellular matrix. Our results show that both PDL and laminin patterns can be used to confine neuronal cells and to control their growth and interconnectivity on Au surfaces, a significant step towards the engineering of artificial neuronal assemblies with well-controlled neuron position and connections. (c) 2009 Elsevier Ltd. All rights reserved.
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