Journal
BIOMATERIALS
Volume 30, Issue 23-24, Pages 4021-4028Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.04.005
Keywords
ECM (extracellular matrix); Cell culture; Adhesion; Cell proliferation
Funding
- NIH STTR [1R41EB005900-01A1]
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Recent studies have shown that extracellular matrix (ECM) substitutes can have a dramatic impact on cell growth, differentiation and function. However, these ECMs are often applied generically and have yet to be developed for specific cell types. In this study, we developed tissue-specific ECM-based coating substrates for skin, skeletal muscle and liver cell cultures. Cellular components were removed from adult skin, skeletal muscle, and liver tissues, and the resulting acellular matrices were homogenized and dissolved. The ECM solutions were used to coat culture dishes. Tissue matched and non-tissue matched cell types were grown on these coatings to assess adhesion, proliferation, maintenance of phenotype and cell function at several time points. Each cell type showed better proliferation and differentiation in cultures containing ECM from their tissue of origin. Although subtle compositional differences in the three ECM types were not investigated in this study, these results suggest that tissue-specific ECMs provide a culture microenvironment that is similar to the in vivo environment when used as coating substrates, and this new culture technique has the potential for use in drug development and the development of cell-based therapies. (C) 2009 Elsevier Ltd. All rights reserved.
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