Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 26, Issue 7, Pages 537-543Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(99)00192-1
Keywords
L-lysine; enzyme sensor; L-lysine-(alpha)-oxidase; screen-printing; fermentation
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Sensors for the determination of L-lysine in samples of fermentation broth have been developed. Low-cost screen-printed sensors comprising a platinum working electrode, an Ag/AgCl pseudo reference and a carbon counter electrode were used as transducers for the enzyme sensors. L-lysine-(alpha)-oxidase from Trichoderma viride has been immobilized by entrapment into a polyurethane hydrogel. Sensors were characterized for L-lysine with respect to pH value, linear range, reproducibility, repeatability, storage and working stability. The sensitivities to other amino acids were also determined. A batch system with two working electrodes, one with immobilized enzyme and one without was adapted for the determination of L-lysine by differential measurements. Good agreement was found between L-lysine concentrations measured by the enzyme sensors and by a conventional amino acid analyzer. (C) 2000 Elsevier Science Inc. All rights reserved.
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