Journal
FERTILITY AND STERILITY
Volume 73, Issue 4, Pages 687-693Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0015-0282(99)00640-8
Keywords
sperm; motility; pH; BufferGel; contraceptive; intracellular pH
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Funding
- NICHD NIH HHS [N01-HD-5-3243] Funding Source: Medline
- NIGMS NIH HHS [GM07231-23] Funding Source: Medline
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Objective: To determine the rate at which mild acidity immobilizes and kills human sperm and to evaluate an acidic microbicide, BufferGel, for sperm immobilization. Design: Controlled in vitro study. Setting: An academic research university and hospital andrology lab. Patient(s): Eight volunteer male sperm donors. Intervention(s): Semen samples were treated with hydrochloric acid (HCl) or BufferGel. Main Outcome Measure(s): Sperm motility was measured by using a computerized automated semen analyzer and video microscopy. Sperm membrane permeability and intracellular pH were measured by using fluorescent techniques. Result(s): In semen acidified with HCl to ptr 4.0, sperm were rapidly immobilized (within 1 min) and were irreversibly immobilized (killed) within 10 minutes. The speed of immobilization and of killing were both linearly proportional to hydrogen ion activity over a pH range of 7.5-4.0. Across the same range, the intracellular pH of human sperm equilibrated to within 0.5 pH units of extracellular pH within 1-2 minutes. BufferGel immobilized sperm significantly faster than HCl from pH 4.0-6.0. Conclusion(s): Exposure to mild acidity rapidly acidifies the intracellular pH of human sperm and is rapidly spermicidal. BufferGel accelerates acid immobilization of sperm.
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