Journal
BIOMATERIALS
Volume 29, Issue 6, Pages 653-661Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2007.10.025
Keywords
electrospinning; peripheral nerve regeneration; contact guidance; neural tissue engineering; Schwarnn cell
Funding
- NHLBI NIH HHS [R01 HL083008, R01 HL083008-02] Funding Source: Medline
- NIBIB NIH HHS [R01 EB003447-05, R01 EB003447, EB003447] Funding Source: Medline
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Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Bungner prior to implantation. Aligned electrospun poly(epsilon-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton. and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Bungner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on two-dimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, myelin-associated glycoprotein (MAG), and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly oriented PCL electrospun fibers. However, upregulation of the myelin-specific gene, PO, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation. (c) 2007 Elsevier Ltd. All rights reserved.
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