Journal
BIOMATERIALS
Volume 29, Issue 30, Pages 4137-4145Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2008.07.011
Keywords
Magnetoliposome; Iron oxide nanoparticles; Confocal fluorescence microscopy; Magnetic cell labeling; Magnetophoresis; Uptake kinetics
Funding
- Ministry of Research in France
- CNRS
- ACI NR [145]
Ask authors/readers for more resources
Interactions of magnetic-fluid-loaded liposomes (MFL) with human adenocarcinoma prostatic cell line PC3 were investigated in vitro. MFL consisted of unilamellar phosphatidylcholine vesicles (mean hydrodynamic diameter close to 180 nm) encapsulating 8-nm nanocrystals of maghemite (gamma-Fe2O3) and sterically stabilized by introducing 5 mol.% of distearylphosphatidylcholine poly(ethylene glycol)2000 (DSPE-PEG(2000)) in the vesicle bilayer. The association processes with living cells, including binding and effective internalization, were followed versus time at two levels. On one hand, the lipid vesicles labeled by I mol.% of rhodamine-marked phosphatidylethanolamine were imaged by confocal fluorescence microscopy. On the other hand, the iron oxide particles associated with cells were independently quantified by magnetophoresis. This allowed modeling of MFL uptake kinetics as a two-step process involving first binding adsorption onto the outer cell membrane followed by subsequent internalization. Capture efficiency was significantly improved by guiding MFL in the near vicinity of the cells by means of a 0.29-T external magnet developing a magnetic field gradient close to 30 mT/mm. Double detection of lipids by fluorescence tracking and of iron oxide by magnetophoresis showed excellent Correlation. This demonstrated that MFL associate with tumor cells as intact vesicle structures which conserve their internal content. (C) 2008 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available