Journal
BIOMATERIALS
Volume 29, Issue 35, Pages 4676-4683Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2008.08.018
Keywords
Neural stem/progenitor cell differentiation; Hydrogel; Growth factor immobilization
Funding
- Ontario Centres of Excellence
- Natural Science and Engineering Research Council (NSERC)
- Canadian Institute of Health Research (CIHR)
- Collaborative Health Research Program (CHRP)
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Neural stem/progenitor cells (NSPCs) hold great promise in regenerative medicine; however, controlling their differentiation to a desired phenotype within a defined matrix is challenging. To guide the differentiation of NSPCs, we first created a cell-adhesive matrix of agarose modified with glycine-arginine-glycine-aspartic acid-serine (GRGDS) and then demonstrated the multi potentiality of NSPCs to differentiate to the three primary cell types of the central nervous system on this matrix: neurons, oligodendrocytes and astrocytes. We then examined whether immobilized platelet derived growth factor AA (PDGF-AA) would promote differentiation similarly to the same soluble factor and found similar percentages of NSPCs differentiated to oligodendrocytes as determined by immunohistochemistry (IHC) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Interestingly, the gene expression of the differentiated oligodendrocytes was similar for 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) but different for myelin oligodendrocyte glycoprotein (MOG) in the presence of soluble PDGF-AA vs. immobilized PDGF-AA. These results demonstrate for the first time, that it is possible to control the differentiation of NSPCs, and specifically to oligodendrocytes, in cell-adhesive matrices with immobilized PDGF-AA. (c) 2008 Elsevier Ltd. All rights reserved.
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