The biocompatibility of quantum dot probes used for the targeted imaging of hepatocellular carcinoma metastasis

Semiconductor quantum dots (QDs) have several photo-physical advantages over organic dyes making them good markers in biomedical application. We used CdSe/ZnS QDs with maximum emission wavelength of 590 nm (QD590) linked to alpha-fetoprotein (AFP) monoclonal antibody (Ab) to detect AFP in cytoplasm of human hepatocellular carcinoma (HCC) cell line HCCLM6. For the in vivo studies, we used QD-AFP-Ab probes for targeted imaging of human HCC xenograft growing in nude mice by injecting them into the tail vein. In addition, the cytotoxicity in vitro, the acute toxicity in vivo, the hemodynamics and tissue distribution of these probes were also investigated. The results in vitro and in vivo indicate that our QD-based probes have good stability, specificity and biocompatibility for ultrasensitive fluorescence imaging of molecular targets in our liver cancer model system. (C) 2008 Elsevier Ltd. All rights reserved.