Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 55, Issue 2-3, Pages 138-144Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/S1011-1344(00)00036-1
Keywords
manganese; oxygen-evolving complex; Chlamydomonas reinhardtii; Scenedesmus obliquus; photosynthesis
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Photosystem II (PS II) membrana particles are particularly well suited for various types of spectroscopic investigations on the PS II manganese complex. Here we present: ( I) a preparation protocol for PS II membrane particles of higher plants, which yields exceptionally high oxygen-evolution activity due to the use of glycinebetaine as a PS II-stabilizing agent; (2) preparation protocols for highly active PS II membrane particles for the green algae Scenedesmus obliquus and Chlamydomonas reinhardtii; (3) a determination of pH dependence of oxygen evolution for spinach and Scenedesmus; (4) a comparison of the EPR multiline signal observed in the S-2-state of green algae and higher plants of PS II membrane particles. A clearly broader type of multiline EPR signal is observed in green algae. (C)2000 Elsevier Science S.A. All rights reserved.
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