Journal
HUMAN REPRODUCTION
Volume 15, Issue 4, Pages 857-860Publisher
OXFORD UNIV PRESS
DOI: 10.1093/humrep/15.4.857
Keywords
IVF; Percoll; PureSperm (R); sperm morphology; swim-up
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Silane-coated silica particles (PureSperm(R)) were evaluated as an alternative to Percoll for gradient separation of spermatozoa, for use in assisted reproduction. Recovery of motile and morphologically normal spermatozoa after using a four-layer Percoll and a two- and four-layer PureSperm(R) gradient respectively was recorded. In-vitro fertilization (IVF) results after using PureSperm(R) for the sperm preparation were also evaluated. No difference in sperm recovery or sperm motility was found when comparing the use of Percoll and the four-layer gradient of PureSperm(R). When using a two-layer PureSperm(R) gradient, motility was significantly decreased (P < 0.05) compared to Percoll. Normal sperm morphology increased from 8-17.2% after using Percoll and to 12.7% and 11.4% after using a four-layer and a two-layer PureSperm(R) gradient respectively. All gradient preparations showed a significant decrease in the teratozoospermia index compared to the ejaculate (P < 0.01). No significant differences in IVF results regarding fertilization and pregnancy rates were found when PureSperm(R) or the swim-up technique were used for the sperm preparation. PureSperm(R) seems to be an acceptable alternative to Percoll but although the percentage of sperm recovery was higher after PureSperm(R) we still recommend the swim-up technique to be the first choice, as a higher percentage of progressive motile spermatozoa is obtained without using other chemicals than IVF culture medium.
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