Journal
BIOMASS & BIOENERGY
Volume 69, Issue -, Pages 175-182Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biombioe.2014.07.018
Keywords
Jerusalem artichoke; Inulin; Enzymatic hydrolysis; Optimization; Response surface methodology (RSM); ABE fermentation
Funding
- Sand Plains Community Development Fund
- Agriculture Canada
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canada Foundation for Innovation (CFI) [27300]
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In this study, a central composite design and response surface methodology were used to study the effect of various enzymatic hydrolysis variables (temperature, pH, substrate concentration and enzyme loading) on the enzymatic hydrolysis of Jerusalem artichoke-derived inulin. It was found that a quadratic model was able to predict inulin conversion as a function of all four investigated factors. The model was confirmed through additional experiments and via analysis of variance (ANOVA). Subsequently, numerical optimization was used to maximize the inulin conversion (94.5%) of Jerusalem artichoke powder within the experimental range (temperature of 48 degrees C, pH of 4.8, substrate concentration of 60 g l(-1), and enzyme loading of 10 units a g(substrate)(-1) for 24 h). The enzymatic hydrolyzate of Jerusalem artichoke was fermented via solventogenic clostridia to acetone-butanol-ethanol (ABE). An ABE yield of 0.33 g(Solvent) g(sugar)(-1) and an overall fermentation productivity of 0.25 g l(-1) h(-1) were obtained indicating the suitability of this feedstock for fermentative ABE production. (C) 2014 Elsevier Ltd. All rights reserved.
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