Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 48, Issue 4, Pages 481-485Publisher
HISTOCHEMICAL SOC INC
DOI: 10.1177/002215540004800405
Keywords
FluoroNanogold; immunocytochemistry; correlative microscopy; fluorescence microscopy; electron microscopy; ultrathin cryosections; myeloperoxidase; human neutrophils
Categories
Funding
- NICHD NIH HHS [HD35121] Funding Source: Medline
Ask authors/readers for more resources
We applied a fluorescent ultrasmall immunogold probe, FluoroNanogold (FNG), to immunocytochemistry on ultrathin cryosections. FNG has the properties of both a fluorescent dye-conjugated antibody for fluorescence microscopy and a gold particle-conjugated antibody for electron microscopy. Therefore, this bifunctional immunoprobe permits correlative microscopic observation of the same cell profiles labeled in a single labeling procedure by these two imaging methods. We demonstrate the utility of FNG as a secondary antibody for immunocytochemical labeling of myeloperoxidase (a marker protein for azurophilic granules) in ultrathin cryosectioned human neutrophils. Its detection requires high spatial resolution because neutrophils contain many cytoplasmic granules. There was a one-to-one relationship between fluorescent structures labeled with FNG and organelle profiles labeled with the same silver-enhanced FNG in ultrathin cryosections. Use of FNG immunocytochemistry on ultrathin cryosections is an ideal methodology for high-resolution correlative fluorescence and electron microscopy and can provide unique information that may be difficult to obtain with a single imaging regimen.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available