Journal
ANALYTICA CHIMICA ACTA
Volume 882, Issue -, Pages 68-75Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.04.012
Keywords
Hypochlorous acid; Fluorescent probe; Boron dipyrromethene; Bioimaging
Categories
Funding
- Ministry of Science and Technology (Taiwan) [MOST 103-2113-M-009-005]
- National Chiao Tung University
Ask authors/readers for more resources
A BODIPY-based fluorescent probe, HBP, was developed for the detection of hypochlorous acid based on the specific hypochlorous acid-promoted oxidative intramolecular cyclization of heterocyclic hydrazone in response to the amount of HOCl. The reaction is accompanied by a 41-fold increase in the fluorescent quantum yield (from 0.004 to 0.164). The fluorescence intensity of the reaction between HOCl and HBP is linear in the HOCl concentration range of 1-8 mu M with a detection limit of 2.4 nM (S/N = 3). Confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe HBP could be used as an effective fluorescent probe for detecting HOCl in living cells. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available