4.7 Article

Fermentation and purification of cellulase from a novel strain Rhizopus stolonifer var. reflexus TP-02

Journal

BIOMASS & BIOENERGY
Volume 36, Issue -, Pages 366-372

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biombioe.2011.11.003

Keywords

Cellulase; Identification; Rhizopus stolonifer var. reflexus; Purification PAGE; Zymogram

Funding

  1. Natural Science Foundation of Anhui Province [KJ2007A018]

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The morphology, cultural characteristics and internal transcribed spacer's (ITS)-5.85 rDNA analysis of TP-02 were applied to identify a strain of filamentous fungus Rhizopus TP-02 with high activity of cellulase. The result of that showed that the novel strain could be identified as Zygomycotina, Phycomycetes, Mucorales, Mucoraceae, Rhizopus stolonifer var. reflexus. The filter paper activity and CMC activity of the strain TP-02 could achieve 7.56 IU/mL and 28.5 IU/mL respectively after 96 h fermentation. During the tests of purification procedure, three types of Endo-beta-1, 4-glucanases and two different molecular weight of beta-1, 4-glucosidases were detected from the crud enzyme produced by the strain. (C) 2011 Elsevier Ltd. All rights reserved.

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