Journal
BIOMASS & BIOENERGY
Volume 35, Issue 5, Pages 2053-2058Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biombioe.2011.01.047
Keywords
SO2 catalyzed; Steam-pretreated corn stover; Polyethylene glycol; Saccharification; Cellulase; beta-Glucosidase
Funding
- National Major Basic Research Program of China [2007CB707801]
- National Natural Science Foundation of China [31070513]
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Corn stover is a renewable, low cost and abundant feedstock in China. Its effective utilization is crucial for providing bioenergy, releasing environmental pollution and increasing farmers' income. This aim of this study was to obtain the efficient saccharification of SO2 catalyzed steam-exploded corn stover (SSECS) by polyethylene glycol (PEG) addition. According to the results, adding PEG6000 could lower the enzyme loading by 33.3%. With 20% solid loading, the highest glucose concentration of 102 g L-1 and 91.3% saccharification yield were obtained using 30 CBU (g glucan)(-1) beta-glucosidase and 10 FPU (g glucan)(-1) cellulase in presence of PEG6000. In addition, protein and enzyme activities assays in the supernatants revealed that PEG could facilitate the desorption of enzyme protein from lignocellulose. These indicated that PEG addition not only can enhance enzymatic saccharification at high substrate concentration, but also can improve enzyme recycling by reducing the enzyme activity loss caused by adsorption during the hydrolysis. (C) 2011 Elsevier Ltd. All rights reserved.
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