Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
Volume 125, Issue 4, Pages 497-502Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S1095-6433(00)00181-1
Keywords
isolated digestive-gland cells; cell suspensions; abalone; Haliotis kamtschatkana
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We describe a method for preparation and maintenance of isolated digestive-grand cells in the abalone, Haliotis kamtschatakana. Viability of the isolated cells was confirmed by the fact that 18 h after preparation the cells exhibited less than 5% staining with trypan blue and actively synthesized glycogen following the addition of glucose substrate. Use of the method in a 15-month study of metabolic activity of the digestive gland of H. kamtschatkana showed significant differences in oxygen consumption of isolated-cell preparations correlated with seasonal differences in somatic and gametogenetic growth, and with relative size of the digestive gland. (C) 2000 Elsevier Science Inc. All rights reserved.
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