Journal
EMBO JOURNAL
Volume 19, Issue 7, Pages 1494-1504Publisher
OXFORD UNIV PRESS
DOI: 10.1093/emboj/19.7.1494
Keywords
Golgi; membrane transport; NSF; SNAREs
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Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimide-sensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi V-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.
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