Journal
GENE
Volume 246, Issue 1-2, Pages 179-185Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-1119(00)00083-4
Keywords
Cre-recombinase; G418 resistance; HA; PCR-tagging; loxP; MYC; polyhistidine; VSV
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Gene tagging can be achieved by homologous recombination in yeast. The kan(t) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly. (C) 2000 Elsevier Science B.V. All rights reserved.
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