Journal
ANALYTICA CHIMICA ACTA
Volume 875, Issue -, Pages 92-98Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.02.022
Keywords
Colorimetric biosensor; Gold nanoparticle; alpha-Glucosidase; Inhibitor
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Funding
- National Natural Science Foundation of China [61275085, 31100560]
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A colorimetric sensor has been developed in this work to sensitively detect alpha-glucosidase activity and screen alpha-glucosidase inhibitors (AGIs) utilizing unmodified gold nanoparticles (AuNPs). The sensing strategy is based on triple-catalytic reaction triggered by alpha-glucosidase. In the presence of alpha-glucosidase, aggregation of AuNPs is prohibited due to the oxidation of cysteine to cystine in the system. However, with addition of AGIs, cysteine induced aggregation of AuNPs occurs. Thus, a new method for alpha-glucosidase activity detection and AGIs screening is developed by measuring the UV-vis absorption or visually distinguishing. A well linear relation is presented in a range of 0.0025-0.05 U mL(-1). The detection limit is found to be 0.001 U mL(-1) for alpha-glucosidase assay, which is one order of magnitude lower than other reports. The IC50 values of four kinds of inhibitors observed with this method are in accordance with other reports. The using of unmodified AuNPs in this work avoids the complicated and time-consuming modification procedure. This simple and efficient colorimetric method can also be extended to other enzymes assays. (C) 2015 Published by Elsevier B.V.
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