Journal
NEUROREPORT
Volume 11, Issue 5, Pages 1081-1084Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200004070-00035
Keywords
blood-brain barrier; cortical neurons; endothelial cells; extracellular matrix; occludin
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We report that extracellular matrix and neurons modulate the expression of occludin, one of the main components of tight junctions, by rat brain endothelial cells (RBE4.B). Of the three extracellular matrix proteins which we tested (collagen I, collagen IV, and laminin), collagen IV stimulated at the best the expression of occludin mRNA. The corresponding protein, however, was not synthesized. Significant amounts of occludin accumulated only when RBE4.B cells were cultured on collagen IV-coated inserts, in the presence of cortical neurons, plated on laminin-coated companion wells. Finally, occludin segregated at the cell periphery, only when endothelial cells were cocultured with neurons for greater than or equal to 1 week. NeuroReport 11:1081-1084 (C) 2000 Lippincott Williams & Wilkins.
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