Journal
FEMS MICROBIOLOGY LETTERS
Volume 185, Issue 2, Pages 163-168Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2000.tb09056.x
Keywords
polyurethane; polyurethanase; sequence; lipase; Pseudomonas
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A gene (pueA, polyurethane esterase A) encoding an extracellular polyurethanase (PueA) was cloned from Pseudomonas chlororaphis into Escherichia coli. The enzyme secreted from E. coli showed esterase activity when assayed with p-nitrophenyl acetate. Subcloning of a 3.2-kb SalI-EcoRI fragment into a T7 RNA polymerase expression vector (pT7-6) produced a S-35-labeled protein of 65 kDa. Nucleotide sequencing of pueA showed an open reading frame encoding a 65-kDa protein of 617 amino acid residues, with the serine hydrolase consensus sequence GXSXG. PueA was over-expressed using the pT7-6 vector transformed into E. coli BL21(DE3) and was purified in one step using Sephadex G-75. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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