Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1457, Issue 3, Pages 106-117Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0005-2728(00)00070-0
Keywords
magnetic circular dichroism; bacteriochlorophyll a; coordination state; light-harvesting complex 1; n-octyl-beta-D-glucopyranoside (beta-OG) treatment
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Magnetic circular dichroism (MCD) and absorption spectra of metal bacteliochlorin complexes have been measured on bacteriochlorophyll (BChl) a in various solvents and different forms of light-harvesting complexes 1 (LH1 complexes). In hydrophilic organic solvents, the MCD intensity of the Q(y)(0-0) transition of BChl a was sensitive to the wavelength of absorption maximum of Q(x)(0-0), and the ratio of MCD Q(y)(0-0) intensity to the dipole strength (B/D) was inversely proportional to the difference in energy between the Q(x)(0-0) and Q(y)(0-0). The similar correlation has been observed in metal chlorin derivatives as previously reported. The correlation depends on the coordination number of the Mg atom in BChl a and the molecules ligating to it. In a hydrophobic solvent such as carbon tetrachloride (CCl4), however, the correlation did trot hold because of the existence of aggregates. Hence, the col relation between the values of B/D and the energy difference can be used to estimate the type and number of the molecules ligated to the Mg atom and to disclose the existence of aggregated pigments. We further apply the correlation to the LH 1 complex treated with n-octyl beta-D-glucopyranoside. (C) 2000 Elsevier Science B.V. All rights reserved.
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