Journal
BIOMARKERS
Volume 17, Issue 3, Pages 231-239Publisher
INFORMA HEALTHCARE
DOI: 10.3109/1354750X.2012.657246
Keywords
Nephrotoxicity; renal; biomarker; exosome; urine
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Funding
- Medical Research Council (MRC) (Centre for Drug Safety Science) [G0700654]
- MRC [G0700654] Funding Source: UKRI
- Medical Research Council [G0700654B, G0700654] Funding Source: researchfish
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Context: MiRNAs have been reported to represent sensitive and translational biomarkers of organ injury Objective: To validate the methodologies for the isolation and quantification of a miRNAs from none-invasive biofluids Methods: Commercially available miRNA isolation kits and qPCR was utilised for determination of analyte sensitivity, stability, recovery, and precision Results: qPCR was highly precise and sensitive for endogenous miRNA quantification (miR-194, LLQ; 0.1 pM). Intra and inter-assay variation remained low (<12%). Variable recovery (54-89%) was controlled for by internal synthetic standards (C. elegans Lin-4) Conclusions: Translational endogenous miRNAs reflective of renal injury represent stable analytes with a large dynamic range that can be quantified easily in most laboratories
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