Journal
BIOMARKERS
Volume 17, Issue 1, Pages 48-55Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/1354750X.2011.637180
Keywords
Chronic renal failure; metabonomics; serum; rats; UPLC Q-TOF MS
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Funding
- National Scientific Foundation of China [81001622]
- Ministry of Science and Technology of the People's Republic of China [2011ZX09401-308-34]
- Shaanxi Provincial Education Department [11JK0678]
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An ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC Q-TOF MS) metabonomics approach was employed to study the serum metabolic profiling of adenine-induced chronic renal failure (CRF) rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the CRF and the normal control groups. Potential biomarkers were screened by using S-plot and were identified by the accurate mass, isotopic pattern and MS/MS fragments information obtained from UPLC Q-TOF MS analysis. Significant differences in the serum level of creatinine, amino acids and LysoPCs were observed, indicating the perturbations of amino acid metabolism and phospholipid metabolism in adenine-induced CRF rats. This research proved that metabonomics is a promising tool for disease research.
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