Journal
BIOMARKERS
Volume 15, Issue 3, Pages 266-276Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/13547500903521810
Keywords
Proteoglycans; cartilage; osteoarthritis; ELISA; biomarkers; aggrecanases; MMPs
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Aggrecan is one of the first proteins to be depleted from articular cartilage in early osteoarthritis. We investigated the molecular differences between matrix metalloproteinase (MMP)- and aggrecanase-mediated aggrecan degradation, as a consequence of their distinct time-dependent degradation profiles. Cartilage degradation was induced by cytokine stimulation in bovine articular cartilage explants and quantified by a dye-binding assay and immunoassays. The size of degradation fragments was analysed by Western blot. Cytokine stimulation resulted in the early release of aggrecanase-mediated aggrecan degradation fragments. In contrast, MMP-mediated aggrecan degradation began only at day 16 and continued to day 21. Western blot analysis showed that glycosylated high-molecular-weight
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