Journal
BIOMACROMOLECULES
Volume 15, Issue 10, Pages 3503-3510Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm5004948
Keywords
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Funding
- Office of Basic Energy Sciences, U.S. DOE
- ARO [W911NF-11-1-0449]
- GK-12 Fellows [DGE-0741714]
- NSF DMR BMAT program [NSF 1207480]
- NSF MRSEC Program [DMR-0820341]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1207480] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1126005] Funding Source: National Science Foundation
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The fabrication of de novo proteins able to self-assemble on the nano- to meso-length scales is critical in the development of protein-based biomaterials in nanotechnology and medicine. Here we report the design and characterization of a protein engineered coiled-coil that not only assembles into microfibers, but also can bind hydrophobic small molecules. Under ambient conditions, the protein forms fibers with nanoscale structure possessing large aspect ratios formed by bundles of a-helical homopentameric assemblies, which further assemble into mesoscale fibers in the presence of curcumin through aggregation. Surprisingly, these biosynthesized fibers are able to form in conditions of remarkably low concentrations. Unlike previously designed coiled-coil fibers, these engineered protein microfibers can bind the small molecule curcumin throughout the assembly, serving as a depot for encapsulation and delivery of other chemical agents within protein-based 3D microenvironments
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