Journal
EXPERIMENTAL PARASITOLOGY
Volume 95, Issue 1, Pages 45-53Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/expr.2000.4503
Keywords
Lutzomyia longipalpis; Phlebotomine sand flies; blood-feeding insects; salivary glands; adenosine deaminase (EC 3.5.4.4); adenosine; inosine
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In the process of sequencing a subtracted cDNA library from the salivary glands of the sand fly Lutzomyia longipalpis, we identified a cDNA with similarities to gene products of the adenosine deaminase family. Prompted by this cDNA finding, we detected adenosine deaminase activity at levels of 1 U/mg protein in salivary gland homogenates. The activity was significantly reduced following a blood meal indicating its apparent secretory fate. The native enzyme has a K-m of similar to 10 mu M, an isoelectric pH between 4.5 and 5.5, and an apparent molecular weight of 52 kDa by size exclusion chromatography. The possible role of this enzyme, which converts adenosine to inosine, in the feeding physiology of L. longipalpis is discussed.
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