Journal
EUROPEAN JOURNAL OF BIOCHEMISTRY
Volume 267, Issue 9, Pages 2760-2767Publisher
WILEY
DOI: 10.1046/j.1432-1327.2000.01297.x
Keywords
cGMP; cyclic nucleotide; feedback regulation; phosphodiesterase; protein phosphorylation
Categories
Funding
- NIDDK NIH HHS [DK40029] Funding Source: Medline
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In addition to its cGMP-selective catalytic site, cGMP-binding cGMP-specific phosphodiesterase (PDES) contains two allosteric cGMP-binding sites and at least one phosphorylation site (Ser92) on each subunit [Thomas, M.K., Francis, S.H. & Corbin, J.D. (1990) J. Biol. Chem. 265, 14971-14978]. Tn the present study, prior incubation of recombinant bovine PDES with a phosphorylation reaction mixture [cGMP-dependent protein kinase (PKG) or catalytic subunit of cAMP-dependent protein kinase (PKA), MgATP, cGMP, 3-isobutyl-1-methylxanthine], shown earlier to produce Ser92 phosphorylation, caused a 50-70% increase in enzyme activity and also increased the affinity of cGMP binding to the allosteric cGMP-binding sites. Both effects were associated with increases in its phosphate content up to 0.6 mol per PDES subunit. Omission of any one of the preincubation components caused loss of stimulation of catalytic activity. Addition of the phosphorylation reaction mixture to a crude bovine lung extract, which contains PDE5, also produced a significant increase in cGMP PDE catalytic activity. The increase in recombinant PDES catalytic activity brought about by phosphorylation was time-dependent and was obtained with 0.2-0.5 mu M PKG subunit, which is approximately the cellular level of this enzyme in vascular smooth muscle. Significantly greater stimulation was observed using cGMP substrate concentrations below the K-m value for PDES, although stimulation was also seen at high cGMP concentrations. Considerably higher concentration of the catalytic subunit of PKA than of PKG was required For activation. There was no detectable difference between phosphorylated and unphosphorylated PDES in median inhibitory concentration for the PDES inhibitors, sildenafil, or zaprinast 3-isobutyl-1-methylxanthine. Phosphorylation reduced the cGMP concentration required for half-maximum binding to the allosteric cGMP-binding sites from 0.13 to 0.03 mu M. The mechanism by which phosphorylation of PDES by PKG could be involved in physiological negative-feedback regulation of cGMP levels is discussed.
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