Journal
JOURNAL OF ANTIBIOTICS
Volume 53, Issue 5, Pages 484-495Publisher
JAPAN ANTIBIOT RES ASSN
DOI: 10.7164/antibiotics.53.484
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Funding
- NIAID NIH HHS [AI 20264, AI 38947] Funding Source: Medline
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The role of two thioesterase genes in the premature release of polyketide synthase intermediates during rifamycin biosynthesis in the Amycolatopsis mediterranei S699 strain was investigated. Creation of:an in-frame deletion in the rifR gene led to a 30 similar to 60% decrease in the production of both rifamycin B by the S699 strain or a series of tetra- to decaketide shunt products of polyketide chain assembly by the rifF strain. Since a similar percentage decrease was seen in both genetic backgrounds, we conclude that the RifR thioesterase 2 is not involved in premature release of the carbon chain assembly intermediates. Similarly, fusion of the Saccharopolyspora erythraea DEBS3 thioesterase 1 domain to the C-terminus of the RifE PKS subunit did not result in a noticeable increase in the amount of the undecaketide intermediate formed nor in the amounts of the tetra- to decaketide shunt products. Hence, premature release of the carbon chain assembly intermediates is an unusual property of the Rif PKS itself.
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