Journal
JOURNAL OF IMMUNOLOGY
Volume 164, Issue 9, Pages 4543-4550Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.164.9.4543
Keywords
-
Categories
Funding
- NCI NIH HHS [CA 70218] Funding Source: Medline
- NEI NIH HHS [EY 09638] Funding Source: Medline
Ask authors/readers for more resources
The mucosa of the conjunctiva is an important site of entry for environmental Ags as well as Ags emanating from the eye itself. However, very little is known about T cell recognition of Ag introduced through this important mucosal site. We have characterized the in vivo process of CD4 T cell recognition of Ag delivered via the conjunctival mucosa, Application of soluble OVA to the conjunctiva of BALB/c mice induced potent T cell tolerance, APC-presenting OVA peptide in vivo was only found in the submandibular lymph node and not in other lymph nodes, spleen, or nasal-associated lymphoid tissue. Similarly, in TCR transgenic DO11.10 adoptive transfer mice, OVA-specific CD4(+) T cell clonal expansion was only observed in the submandibular lymph node following conjunctival application of peptide. These experiments thus define a highly specific lymphatic drainage pathway from the conjunctiva, OVA-specific T cell clonal expansion peaked at day 3 following initiation of daily OVA administration and gradually declined during the 10-day treatment period, but remained elevated compared with nontreated adoptive transfer mice, During this period, the T cells expressed activation markers, and proliferated and secreted IL-2 in vitro in response to OVA stimulation. In contrast, these cells were unable to clonally expand in vivo, or proliferate in vitro following a subsequent OVA/CFA immunization. These results suggest that Ag applied to a mucosal site can be efficiently presented in a local draining lymph node, resulting in initial T cell priming and clonal expansion, followed by T cell anergy, The Journal of Immunology, 2000, 164: 4543-4550.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available