Journal
BIOMACROMOLECULES
Volume 14, Issue 8, Pages 2657-2666Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm4005194
Keywords
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Funding
- The Norwegian Research Council [182695-I40, 217708/O10]
- FMC Biopolymer
- AlgiPharma AS
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The polysaccharide alginate is produced by brown algae and some bacteria and is composed of the two monomers, beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G). The distribution and composition of M/G are important for the chemical-physical properties of alginate and result from the activity of a family of mannuronan C-5 epimerases that converts M to G in the initially synthesized polyM. Traditionally, G-rich alginates are commercially most interesting due to gelling and viscosifying properties. From a library of mutant epimerases we have isolated enzymes that introduce a high level of G-blocks in polyM more efficiently than the wild-type enzymes from Azotobacter vinelandii when employed for in vitro epimerization reactions. This was achieved by developing a high-throughput screening method to discriminate between different alginate structures. Furthermore, genetic and biochemical analyses of the mutant enzymes have revealed structural features that are important for the differences in epimerization pattern found for the various epimerases.
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