Journal
BIOMACROMOLECULES
Volume 13, Issue 12, Pages 3938-3948Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm301110s
Keywords
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Funding
- NIH [EB000490]
- AFOSR [FA9550-09-1-0717, FA9550-10-1-0275]
- DOE [DE-SC0004791]
- NSF [DMR-0805197, CHE-1011937]
- U.S. Department of Energy, Basic Energy Sciences, Office of Science [DE-AC02-06CH11357]
- National Center for Research Resources from National Institutes of Health [5P41RR007707]
- National Institute of General Medical Sciences from National Institutes of Health [8P41GM103543]
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Drag line silk from orb-weaving spiders is a copolymer of two large proteins, major ampullate spidroin 1 (MaSp1) and 2 (MaSp2). The ratio of these proteins is known to have a large variation across different species of orb-weaving spiders. NMR results from gland material of two different species of spiders, N. clavipes and A. aurantia, indicates that MaSp1 proteins are more easily formed into beta-sheet nanostructures, while MaSp2 proteins form random coil and helical structures. To test if this behavior of natural silk proteins could be reproduced by recombinantly produced spider silk mimic protein, recombinant MaSp1MaSp2 mixed fibers as well as chimeric silk fibers from MaSp1 and MaSp2 sequences in a single protein were produced based on the variable ratio and conserved motifs of MaSp1 and MaSp2 in native silk fiber. Mechanical properties, solid-state NMR, and XRD results of tested synthetic fibers indicate the differing roles of MaSp1 and MaSp2 in the fiber and verify the importance of postspin stretching treatment in helping the fiber to form the proper spatial structure.
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