4.7 Article

Surface Charge Affects Cellular Uptake and Intracellular Trafficking of Chitosan-Based Nanoparticles

Journal

BIOMACROMOLECULES
Volume 12, Issue 7, Pages 2440-2446

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/bm101482r

Keywords

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Funding

  1. National Nature Science Foundation of China [50 703 043]
  2. Chinese Academy of Sciences [KJCX2-YW-M02]
  3. 973 Program [2009CB30305]
  4. National ST Major Project [2009 ZX09503-027]

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Chitosan-based nanoparticles (NPs) are widely used in drug delivery, device-based therapy, tissue engineering, and medical imaging. In this aspect, a clear understanding of how physicochemical properties of these NPs affect the cytological response is in high demand. The objective of this study is to evaluate the effect of surface charge on cellular uptake profiles (rate and amount) and intracellular trafficking. We fabricate three kinds of NPs (similar to 215 nm) with different surface charge via SPG membrane emulsification technique and deposition method. They possess uniform size as well as identical other physicochemical properties, minimizing any differences between the NPs except for surface charge. Moreover, we extend our research to eight cell lines, which could help to obtain a representative conclusion. Results show that the cellular uptake rate and amount are both positively correlated with the surface charge in all cell line. Subsequent intracellular trafficking indicates that some of positively charged NPs could escape from lysosome after being internalized and exhibit perinuclear localization, whereas the negatively and neutrally charged NPs prefer to colocalize with lysosome. These results are critical in building the knowledge base required to design chitosan-based NPs to be used efficiently and specifically.

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