Journal
BIOPHYSICAL JOURNAL
Volume 78, Issue 5, Pages 2435-2440Publisher
BIOPHYSICAL SOCIETY
DOI: 10.1016/S0006-3495(00)76787-7
Keywords
-
Categories
Ask authors/readers for more resources
The membrane/water partition coefficients, K, of 15 electrically neutral (non-charged or zwitterionic) detergents were measured with phospholipid vesicles by using isothermal titration calorimetry, and were compared to the corresponding critical micellar concentrations, cmc. The detergents measured were oligo(ethylene oxide) alkyl ethers (CmEOn with m = 10/ n = 3, 7 and m = 12/n = 3...8); alkylglucosides (octyl, decyl); alkylmaltosides (octyl, decyl, dodecyl); diheptanoylphosphatidylcholine; Tritons (X-100, X-114) and CHAPS. A linear relation between the free energies of partitioning into the membrane and micelle formation was found such that K . CMC similar to 1. The identity K . CMC = 1 was used to classify detergents with respect to their membrane disruption potency. Strong detergents are characterized by K . CMC ( 1 and solubilize lipid membranes at detergent-to-lipid ratios X-b < 1 (alkylmaltosides, tritons, heptaethylene glycol alkyl ethers), Weak detergents are characterized by K . CMC > 1 and accumulate in the membrane- to detergent-to-lipid ratios X-b > 1 before the bilayer disintegrates (alkylglucosides, pentaethylene glycol dodecyl ether).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available