Journal
BIOMACROMOLECULES
Volume 12, Issue 6, Pages 2275-2284Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm200358r
Keywords
-
Funding
- NIH [R01 HG002918-01]
Ask authors/readers for more resources
Protein-based polymers are increasingly being used in biomaterial applications because of their ease of customization and potential monodispersity. These advantages make protein polymers excellent candidates for bioanalytical applications. Here we describe improved methods for producing drag-tags for free-solution conjugate electrophoresis (FSCE). FSCE utilizes a pure, monodisperse recombinant protein, tethered end-on to a ssDNA molecule, to enable DNA size separation in aqueous buffer. FSCE also provides a highly sensitive method to evaluate the polydispersity of a protein drag tag and thus its suitability for bioanalytical uses. This method is able to detect slight differences in drag tag charge or mass. We have devised an improved cloning, expression, and purification strategy that enables us to generate for the first time, a truly monodisperse 20 kDa protein polymer and a nearly monodisperse 38 kDa protein. These newly produced proteins can be used as drag tags to enable longer read DNA sequencing by free-solution microchannel electrophoresis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available