Journal
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
Volume 32, Issue 5, Pages 805-815Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1006/jmcc.2000.1122
Keywords
cardiac hypertrophy; subtractive hybridization; gene induction; pressure overload; calcineurin
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Funding
- NHLBI NIH HHS [5 R29 HL57970-02] Funding Source: Medline
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Cardiac hypertrophy is associated with both adaptive and adverse changes in gene expression. To identify genes regulated by pressure overload, we performed suppressive subtractive hybridization between cDNA from the hearts of aortic-banded (7-day) and sham-operated mice. In parallel, we performed a subtraction between an adult and a neonatal heart, for the purpose of comparing different forms of cardiac hypertrophy. Sequencing more than 100 clones led tu the identification of an array of functionally known (70%) and unknown genes (30%) that are upregulated during cardiac growth, At least nine of those genes were preferentially expressed in both the neonatal and pressure over-load hearts alike, Using Northern blot analysis to investigate whether some of the identified genes were upregulated in the load-independent calcineurin-induced cardiac hypertrophy mouse model, revealed its incomplete similarity with the former models of cardiac growth. (C) 2000 Academic Press.
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