Journal
PLANTA
Volume 210, Issue 6, Pages 1018-1022Publisher
SPRINGER VERLAG
DOI: 10.1007/s004250050712
Keywords
ascorbate; ascorbate oxidase; cell elongation/expansion; Nicotiana (transgenic); protoplast; transgenic tobacco
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When pumpkin (Cucurbita spp., cv. Ebisu Nankin) ascorbate oxidase cDNA was introduced into cultured cells of tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow No. 2) by Agrobacterium-mediated trans formation. the transgenic cells expressed and secreted the recombinant pumpkin ascorbate oxidase into the culture medium. These transgenic cells showed no morphological difference from wild-type cells. However. in the presence of applied hormones protoplasts prepared from the transgenic cells elongated more rapidly than those of wild-type cells. We propose that ascorbate oxidase may play a key role in the regulation of cell expansion perhaps by controlling transport processes through the plasma membrane, but not by affecting the cell wall.
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