4.7 Article

One-Step Analysis of DNA/Chitosan Complexes by Field-Flow Fractionation Reveals Particle Size and Free Chitosan Content

Journal

BIOMACROMOLECULES
Volume 11, Issue 3, Pages 549-554

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/bm901345q

Keywords

-

Funding

  1. Canadian Institutes of Health Research (CIHR)
  2. Natural Sciences and Engineering Research Council of Canada (NSERC)
  3. Fonds quebecois de la recherche sur la nature et les technologies

Ask authors/readers for more resources

The composition of samples obtained upon complexation of DNA with chitosan was analyzed by asymmetrical flow field flow fractionation (AF4) with online UV-visible, multiangle light scattering (MALS), and dynamic light scattering (DLS) detectors. A chitosan labeled with rhodamine B to facilitate UV-vis detection of the polycation was complexed with DNA under conditions commonly used for transfection (chitosan glucosamine to DNA phosphate molar ratio of 5). AF4 analysis revealed that 73% of the chitosan-rhodamine remained free ill the dispersion and that the DNA/chitosan complexes had a broad size distribution ranging from 20 to 160 nm in hydrodynamic radius. The accuracy of the data was assessed by comparison with data from batch-mode DLS and scanning electron microscopy. The AF4 combined with DLS allowed the characterization of small particles that were not detected by conventional batch-mode DLS. The AF4 analysis will prove to be all important tool in the field of gene therapy because it readily provides, ill a single measurement, three important physicochemical parameters of the complexes: the amount Of unbound polycation, the hydrodynamic size of the complexes, and their size distribution.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available