Journal
BIOMACROMOLECULES
Volume 11, Issue 4, Pages 1052-1059Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm100020x
Keywords
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Funding
- National Science Foundation [DMR-0213883]
- Department of Energy [DE-FC26-01BC15317]
- Robert M. Hearin Foundation
- Eastman Chemical Company
- NSF Division of Materials Research/Major Research Instrumentation [0079450]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [0079450] Funding Source: National Science Foundation
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The facile synthesis of polymer-stabilized Au nanoparticles (AuNPs) capable of forming neutral, sterically stable complexes with small interfering RNA (siRNA) is reported. The amine-containing cationic block of poly(N-2-hydroxypropyl methacrylamide(70)-Nock-N-[3-(dimethylamino)propyl] methacrylamide(24)) [P(HPMA(70)-b-DMAPMA(24))] was utilized to promote the in situ reduction of Au3+ to AuNPs and subsequently bind small interfering RNA, while the nonimmunogenic, hydrophilic block provided steric stabilization. The ratio of [DMAPMA](0)/[Au3+](0) utilized in the reduction reaction was found to be critical to the production of polymer-stabilized AuNPs capable of complexing siRNA. Significant protection (similar to 100 times) against nucleases was demonstrated by enzymatic tests, while gene down-regulation experiments indicated successful delivery of siRNA to cancerous cells.
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