Journal
BIOMACROMOLECULES
Volume 10, Issue 7, Pages 1777-1781Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm9001987
Keywords
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Funding
- NSF [CHE-0809832]
- Swiss Government for a fellowship
- California NanoSystems Institute Postdoctoral Award
- Alfred P. Sloan Foundation
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [809832] Funding Source: National Science Foundation
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Maleimide end functionalized polymers for site-selective conjugation to free cysteines of proteins were synthesized using reversible addition-fragmentation chain transfer (RAFT) polymerization. A furan-protected maleimide chain transfer agent (CTA) was employed in the RAFT polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA). Gel permeation chromatography (GPC) with laser light scattering detection indicated that 20000 and 39000 Da polyPEGA had been made with polydispersity indices of 1.25 and 1.36, respectively. The maleimide group on the polymer chain end was exposed by heating the poly(PEGA)s for 4 h. The deprotection efficiency was estimated to be 80 and 60% for poly(PEGA)(20) (kDa) and poly(PEGA)(39) (kDa), respectively. Maleimide-poly(PEGA)s were conjugated to V131C T4 lysozyme (T4L), and the resultant polymer-protein conjugates were characterized by size exclusion chromatography and gel electrophoresis.
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