Journal
BIOMACROMOLECULES
Volume 10, Issue 8, Pages 2207-2212Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm900395h
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Funding
- NSF [CHE-0645793]
- Alfred P. Sloan Foundation
- National Institutes of Health [F31 GM077086-02]
- California NanoSystems Institute
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A pyridyl disulfide end-functionalized polymer with N-acetyl-D-glucosamine pendant side-chains was synthesized by atom transfer radical polymerization (ATRP). The glycopolymer was prepared from a pyridyl disulfide initiator catalyzed by a Cu(I)/Cu(II)/2,2'-bipyridine system in a mixture of methanol and water at 30 degrees C. The final polymer had a number-average molecular weight (M-n) of 13.0 kDa determined by H-1 NMR spectroscopy and a narrow polydispersity index (1.12) determined by gel permeation chromatography (GPC). The pyridyl disulfide end-group was then utilized to conjugate the glycopolymer to a double-stranded short interfering RNA (siRNA). Characterization of the glycopolymer-siRNA by polyacrylamide gel electrophoresis (PAGE) showed 97% conjugation. The activated disulfide polymer was also patterned on gold via microcontact printing. The pyridyl disulfide allowed for ready immobilization of the glycopolymer into 200 mu m sized features on the surface.
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