Journal
BIOMACROMOLECULES
Volume 9, Issue 6, Pages 1669-1677Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bm800200a
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Funding
- NIDCR NIH HHS [DE014672] Funding Source: Medline
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The fang-like jaws of the marine polychaete Nereis virens possess remarkable mechanical properties considering their high protein content and lack of mineralization. Hardness and stiffness properties in the jaw tip are comparable to human dentin and are achieved by extensive coordination of Zn2+ by a histidine-rich protein framework. In the present study, the predominant protein in the jaw tip, Nvjp-1, was purified and characterized by partial peptide mapping and molecular cloning of a partial cDNA from a jaw pulp library. The deduced amino acid sequence revealed an similar to 38 kDa histidine-rich protein rich in glycine and histidine (similar to 36 and 27%, respectively) with no well-defined repetitive motifs. The effects of pH and metal treatment on aggregation, secondary structure, and hydrodynamic properties of recombinant Nvjp-1 are described. Notably, Zn treatment induced the formation of amyloid-like fibers.
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