Journal
ANALYTICA CHIMICA ACTA
Volume 889, Issue -, Pages 82-89Publisher
ELSEVIER
DOI: 10.1016/j.aca.2015.07.018
Keywords
Resonance energy transfer; Nitrogen-doped carbon quantum dots; Alpha fetoprotein; Aminated graphene
Categories
Funding
- Natural Science Foundation of China [21175057, 21375047, 21377046]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
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Nitrogen-doped carbon quantum dots (N-CQDs) with an average diameter of 2 nm were synthesized by carbonization of diethylene triamine pentacetate acid (DTPA). The simple prepared N-CQDs showed excellent electrochemiluminescence (ECL) property and were used as luminophors to fabricate a sandwich-type ECL immunosensor. Aminated graphene (NH2-G) was also synthesized and used as a label of secondary antibody. The labeled NH2-G could effectively quench the ECL of N-CQDs modified on electrodes due to ECL resonance energy transfer (ERET). Immunological recognition which induced ECL quenching enabled the quantitative determination of biomarkers. Alpha fetoprotein (AFP) was selected as a model analyte to investigate the analytical performance of the proposed immunosensor. Under optimal conditions, a good linear relationship between ECL intensity and the logarithm of AFP concentration was obtained in the range of 0.01-100 ng mL(-1) with the detection limit of 3.3 pg mL(-1). The proposed ECL immunosensor showed good stability, acceptable selectivity and reproducibility. (C) 2015 Elsevier B.V. All rights reserved.
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