Journal
CARBOHYDRATE POLYMERS
Volume 42, Issue 1, Pages 23-31Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0144-8617(99)00139-3
Keywords
gum arabic; Acacia senegal; size exclusion chromatography; flow field flow fractionation; light scattering; separation
Categories
Ask authors/readers for more resources
The heterogeneous polysaccharide gum arabic has been characterized using size exclusion chromatography (SEC) and flow field flow fractionation (F4) coupled on-line to multi-angle laser light scattering (MALLS). Two distinct populations have been shown. About 80% of the material consist of highly branched arabinogalactan (AG) units. The rest is mainly composed of heterogeneous arabinogalactan-protein complex (AGP) of high molecular weight. The F4/MALLS method is shown to allow the complete analysis of gum arabic whereas SEC gave only partial information. From light-scattering-based radii of gyration (R-g) and F4-based hydrodynamic radii (R-h) We found that the ratio R-g/R-h is near 0.8 for AGP. This value is reasonably consistent with a globular conformation and gives further evidence in favour of the wattle blossom model proposed by Connolly et al. (C) 2000 Elsevier Science Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available