Journal
BIOLOGY OF REPRODUCTION
Volume 88, Issue 5, Pages -Publisher
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.112.104679
Keywords
fetus; pig; pregnancy; secreted phosphoprotein 1
Categories
Funding
- British Pig Executive
- BBSRC
- BBSRC [BBS/E/D/05191132, BBS/E/D/20221656, BBS/E/D/20310000, BBS/E/D/20211553] Funding Source: UKRI
- MRC [MC_PC_U127592696, MR/K001744/1, MC_U127592696] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/D/20221656, BBS/E/D/20211553, BBS/E/D/20310000, BBS/E/D/05191132] Funding Source: researchfish
- Medical Research Council [MC_PC_U127592696, MR/K001744/1, MC_U127592696] Funding Source: researchfish
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Increased litter size and within-litter uniformity in birth weight would improve pig reproductive efficiency. This study compared the location and gene and protein expression of secreted phosphoprotein 1 in placental and uterine tissues supplying a normally sized and the smallest fetus carried by hyperprolific Large White and Meishan gilts on Days 41-42 of pregnancy. Immunohistochemistry and in situ hybridization showed that the protein and gene encoding secreted phosphoprotein 1 were located in the glandular and luminal epithelium of the endometrium and in the placenta. Secreted phosphoprotein 1 protein levels were higher in glandular epithelium, luminal epithelium, and placenta from Meishan gilts compared to corresponding tissues from hyperprolific Large White gilts. Reverse transcription quantitative PCR demonstrated secreted phosphoprotein 1 mRNA levels were higher in endometrium, but not placenta, from Meishan compared to hyperprolific Large White gilts. In hyperprolific Large White gilts, secreted phosphoprotein 1 protein levels were higher in glandular epithelium and placenta surrounding small fetuses than corresponding tissues supplying normal-sized fetuses. Similarly, in Meishan gilts, secreted phosphoprotein 1 protein levels were higher in luminal epithelium surrounding small compared to normal-sized fetuses. Within hyperprolific Large White, but not Meishan, gilts secreted phosphoprotein 1 mRNA was higher in endometrium surrounding the normal-sized fetus than the control fetus. The contradictory relationship between fetal size and secreted phosphoprotein 1 protein and mRNA in the hyperprolific Large White is intriguing and may reflect breed differences in posttranslational modification. The striking breed differences in secreted phospoprotein 1 expression suggest that SPP1 may be associated with placental efficiency.
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