Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 66, Issue 5, Pages 2238-2242Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.5.2238-2242.2000
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A new three-dimensional (3D)-tracking system with optimized dark-field illumination is presented. It allows simultaneous 3D tracking of several free-swimming microorganisms with diameters of >10 mu m. Resolution limits and illumination efficiencies for different size classes of microorganisms are treated analytically. First applications for 3D tracking of protists are demonstrated.
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