4.4 Article

High-resistance MDCK-C7 monolayers used for measuring invasive potency of tumour cells

Journal

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
Volume 440, Issue 1, Pages 179-183

Publisher

SPRINGER VERLAG
DOI: 10.1007/s004240000282

Keywords

transepithelial electrical resistance; carcinoma; invasion assay; metastasis; Boyden chamber

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We describe an electrophysiological method for evaluating the intrinsic invasive potency of tumour cells using renal cells as an in vitro assay system. A high-resistance clone of Madin-Darby canine kidney cells (MDCK-C7) was grown to confluency in a filter cup. Transepithelial electrical resistance across the MDCK-C7 monolayer was measured in a commercially available electrode chamber. After a transepithelial electrical resistance of about 4,000 Omega cm(2) had been reached, human melanoma or pancreatic carcinoma cells were cocultivated with the MDCK-C7 monolayer. Both carcinoma cell lines induced resistance breakdown measured after 24 h or later depending on seeding density and cell type. Seeding carcinoma cells on the basolateral surface of MDCK-C7 cells caused a similar decrease in transepithelial resistance of the MDCK-C7 monolayer. Resistance breakdown indicates opening of tight junctions prior to tumour cell invasion. In conclusion, the high-resistance MDCK-C7 cell clone could serve as a valuable biological assay system to determine electrically the metastatic potency of tumour cells in vitro.

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